The standing-dead leaf blades of smooth-cordgrass (Spartina alterniflora) have ascospores
of Phaeosphaeria spartinicola in
place by the time they die. The ascospores germinate and pervade
the dead blades, digest much of the blade
material, and convert part of it into ascomata, the exteriorly melanized
spheroids in which the sexual spores are generated. The black
structures seen here are the ascomata of P. spartinicola
mixed with those of Mycosphaerella sp. 2. The spheroidal
ascomata (about 75-150 µm diam) are often clustered together
so closely that they just look like irregular black patches from
surface view -- one has to dig them out with a needle to get a good
look at them. If you look closely at this image, you will see a
few little bright, moist-looking spots in the black areas. These
are the ostioles of mature ascomata, the openings through which the
ascospores are explosively expelled. The shininess at the ostioles is
caused by the tips of the asci, which have pushed through into the air,
in preparation for firing away their ascospores. See Newell & Wasowski,
1995, Estuaries 18:241-249; Newell & Porter, 2000, pp. 159-185, in
Weinstein & Kreeger, Concepts and Controversies in Tidal Marsh Ecology,
Kluwer.