Ergosterol is to fungi what cholesterol is to humans: the major
membrane sterol. The content of ergosterol in the living bodies of
fungi is rather constant, near 5 µg per mg dry mass (with some
exceptions), and ergosterol is nearly absent in other organisms of
decomposition systems. Thus ergosterol can be used as a
biochemical index for living mass of fungi. Ergosterol is easily
measured by liquid chromatography, and detected by its uniquely
strong absorbance of ultraviolet light in the vicinity of 282 nm.
In this graph the chromatogram to the left is for blades of smooth
cordgrass (
Spartina alterniflora) at the
yellow-green senescent stage. The chromatogram to the right is for
naturally-decaying blades of cordgrass about two weeks after
senescence. The word "ergosterol" is shown just above the 282-nm
absorbance peak in each chromatogram. The size of the ergosterol
peak on the right shows the rapidity with which
ascomycetes begin the
pervasion and decay of cordgrass shoots in
the marsh. See Gessner & Newell, 2002, pp 390-408 in C
Hurst et al. (eds) Manual of environmental microbiology. Second edition.
ASM Press, Washington, DC; Newell, 2001, Fungal biomass and productivity,
pp 357-372 in J.H. Paul (ed.), Methods in Microbiology 30. Marine
Microbiology, Academic Press, New York.